At diakinesis (Gr. dia = apart; Gr. kinein = to move) the chromatides that we in a crossing-over are entangling. Since the chromatides deiverge the chiasmata become clearly apparent. Nuclear membrane and nucleoli completely disappear. The spindle arises: from each centrosomes (in animal cells) at the poles microtubules 'grow'. Some microtubules anchor to the kinetochores of the chromosomes. The shortening and thickening of the chromatides proceeds.