Chicken: 48 h

On this page the following view of the embryology in chicken are displayed:
  • Whole mount preparation 48 hours (More...)
  • Cross sections 48 hours; eye and heart formation ( More...)
  • Whole mount preparation 55 hours (More...)
    Stage 48 hrs
    Whole mount preparation 48 hrs
    The position of the embryo with respect to the yolk changes strongly about 48 hours after fertilization. In addition to the head fold of the amnion, also the lateral and caudal amniotic folds begin to form. The outgrowth of the cranial flexure is so strong that the forebrain and hindbrain vesicles become almost located to each other. The cephalic region of the embryo is twisted in such a manner that the left side comes to lie next to the yolk. A second flexure appears at the transition of the head and the body just behind the heart region. The embryo takes now the shape of a C. The head becomes covered by a double fold. These folds definitely establish the first extra embryonic membrane (=outside of the embryo): the amnion membrane. The vitelline (yolk rich) arteries and veins become connected with the extra embryonic circulatory vessels. A few branchial grooves are already visible. The brain divides in to 5 vesicles: telencephalon and diencephalon (both formed by the division of the forebrain vesicle), mesencephalon, metencephalon and myencephalon (both formed by the division of the hindbrain vesicle). The lens placode (placode=plate) will form the lens vesicle, the optic vesicle will become the optic cup and the auditory placode the auditory pit. The heart differentiates in to 4 compartiments: the sinus venosus, connected with the veins, the atrium, the U-shaped ventricle and the bulbus cordis. The atrium and ventricle are well distinguishable in the figure.
    Embryology in chicken 48 hours after fertilization: stained whole-mount preparation
    1 Amnion, 2 Metencephalon, 3 Mesencephalon, 4 Optic cup + lens, 5 Prosencephalon, 6 Otic vesicle, 7 Branchial arches, 8 Atrium , 9 Ventricle, 10 Lateral fold, 11 Lateral mesoderm, 12 Vitelline arteria / vein, 13 Somite, 14 Spine, 15 Tail fold

    Cross section chicken embryo - 48 hours
    A. Ear and eye formation: left whole mount and right cross section (dorsal part oriented to the left here)
    Eye formation embryology chicken
    1 = Myencephalon, 2 = Otic vesicle, 3 = Chorda, 4 = Pharyngeal cavity, 5 = Pharynx, 6 = Optic vesicle, 7 = Lens placode (lens formation), 8 = Optic cup, 9 = Diencephalon
    Schematic representation eye formation embryology chicken
    a, b, c, d : schematical representation of the eye formation overtime; e and f: detail of sections
    1 = Neurectoderm, 2 = Epidermal (surface) ectoderm, 3 = Mesoderm, 4 = Optical vesicle, 5 = Optical cup, 6 = lens, lp = Lens placode (lens formation)
    B. Heart formation: left whole-mount preparation and right cross section (dorsal part oriented to the left)
    Heart formation embryology in chicken
    1 = Spine, 2 = Somite differentiation (sclerotome dermamyotome), 3 = Chorda, 4 = Dorsal aorta arches, 5 = Cranial cardinale vein, 6 = Pharynx, 7 = Pericardial cavity, 8 = Heart formation
    C. Heart formation: left wholemount preparation and right cross section (dorsal part oriented left)
    Heart formation 48 hours embryology in chicken
    1 = Spine, 2 = Dermatome, 3 = Chorda, 4 = Dorsal aorta, 5 = Foregut, 6 = Truncus arteriosus, 7 = Atrium, 8 = Ventricle, 9 = Heart region

    Stage 55 hrs
    Dextro dorsal view at 55 hrs according to Patten
    dextro dorsal view through a 55 hrs embryo of chicken, according to Patten 1920
    Patten, B.M. (1920). The Early Embryology of the Chick. Philadelphia: P. Blakiston's Son and Co.


    last modified: 5 Jan 2012